1998;44:2057–2060. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine. Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, Mouse Cytokine Antibody Array, Panel A Ordering Information, Protocol for near-infrared (NIR) fluorescence detection, Assays for analytes represented in the Mouse Cytokine Array Kit, Panel A, Get Superior Accuracy & Precision with R&D Systems Luminex Assays, Quantikine® QuicKit™ ELISAs from R&D Systems, Chromatin Immunoprecipitation (ChIP) Protocol. The Mouse Cytokine Array Panel A is a rapid, sensitive, and economical tool to simultaneously detect cytokine differences between samples. 200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time. © Copyright 2020 Bio-Techne. Size:1 Each Status:RUO Cat No.:561665. Product Insert(PDF, 116K) 3. Suitable for all liquid sample types Be the first to write a review! MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. Refer to the product datasheet for complete product details. Quick View. RayBio ® C-Series Mouse Cytokine Antibody Array 3 Kit. Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. Protocol for near-infrared (NIR) fluorescence detection 4. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples. Suitable for all liquid sample types. > >> Videos. Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane. Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish. Selected citations for Proteome Profiler Mouse XL Cytokine Array include: There are no citations that match your criteria. Suitable for all liquid sample types. Incubate for one hour on a rocking platform shaker. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet. MSD’s cytokine and chemokine kits can quantify up to 10 analytes using less than 25 ml of sample and providing industry-leading sensitivity and dynamic range. - Find MSDS or SDS, a COA, data sheets and more information. Mouse Cytokine Antibody Array (Membrane, 144 Targets) (Y0061) - Antibody array is a specific form of protein microarray. Detects 62 Mouse Cytokines. R&D Systems Mouse Cytokine Array, Panel A (Catalog # ARY006) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 40 different cytokines, chemokines, and more in a single sample. Prior to the assay, cells were stimulated by 10 pg/ml recombinant murine IL-1β protein (PeproTech) for 24 h. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample. The assay service provided by Eve Technologies on their Mouse Cytokine Array / Chemokine Array 44-Plex facilitates research on immune response, inflammatory disease, allergy, irritable bowel disease (IBD), sepsis and cancer. Mouse Cytokine Array C3. Size:1 Each Status:RUO Cat No.:566927. Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure). Which Brands are Currently Available on bio-techne.com? The relative expression levels of 40 mouse cytokines can be determined without performing numerous immunoassays. Lysates from Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (200 µg lysate, 10 minute exposure). Wrap the membrane and sheet protector in plastic wrap. The Q-Plex™ Mouse Cytokine – Inflammation (14-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. I strongly recommend it. High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation, YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells, Adrenergic Blockade Promotes Maintenance of Dormancy in Prostate Cancer Through Upregulation of GAS6, Sestrin2 modulates cardiac inflammatory response through maintaining redox homeostasis during ischemia and reperfusion, Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages, Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity, NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet, Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion, Dual microglia effects on blood brain barrier permeability induced by systemic inflammation, Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer, Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice, Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway, Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung, Platelet GPIb? 3/29/2017 Mouse Cytokine Array - For BALF Samples A R We used mouse BAL fluid samples from control and treated mouse to compare for altered cytokine response. FAX 612 656 4400. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Quantibody® Mouse Cytokine Array 4000 Kit. Citations are publications that use Bio-Techne products. All Rights Reserved. The signal produced is proportional to the amount of analyte bound. Mouse Cytokine Array Q1. Detects 96 Mouse Cytokines. Suitable for all liquid sample types. Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Wash each well of the 4-Well Multi-dish with 1X Wash Buffer. Our high-quality reagents help ensure the accuracy of your results. R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028). Place the array membrane on a plastic sheet protector. Stall A, Sun Q, Varro R, et al. 1 - 18 of 18 Captured proteins are visualized using chemiluminescent or Laser Scanner. If you are doing Western blotting, you can do this easily. We offer a 100% guarantee on all our products. Screen and compare protein expression levels with high specificity and sensitivity (pg/ml levels). Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. No specialized equipment is necessary 1. Plasma cytokine levels of B6 and BALB apoE-/- mice were analyzed using mouse cytokine arrays from RayBiotech, which contain 144 different anti-cytokine antibodies and positive and negative controls. Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. Q-Plex plates are built by adsorbing 14 distinct capture antibodies in a defined array to the bottom of each well. Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. R&D Systems and Tocris Bioscience products are available to purchase through bio-techne.com. Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6. Quantibody combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array. USA, TEL 612 379 2956 You can also request quotes for ProteinSimple branded instruments and Simple Plex Assays. Samples / Cost: 8 / $750.43, 22 / $1,350.77, 50 / $2,401.38. Wash each array membrane 3 times with 1X Wash Buffer in a separate container. - Find MSDS or SDS, a COA, data sheets and more information. MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples. Assays for analytes represented in the Mouse Cytokine Array Kit, Panel A Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Cover the membrane with the top sheet of the plastic protector. Clin Chem. Besides the ability to detect protein expression, RayBiotech’s system is a more accurate reflection of active cytokine levels because it only detects secreted cytokines, This product is designed with 96 TaqMan Gene Expression Assays loaded in singletons into the array Upon receipt, store it immediately at the temperature recommended below. Do not use past expiration date. Toll-free 800 343 7475 Audio Showcase: Proteome Profiler™ 96 Microplate-based Antibody Arrays. The Mouse Cytokine Chemokine 36-Plex ProcartaPlex 1A Panel enables the study of immune function by analyzing 36 protein targets in a single well using Luminex xMAP technology. Abstract LB77. Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Magnetic Bead–Based Assays detect and measure levels of 46 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples. Place the array membrane in the diluted Streptavidin-HRP solution. All analyses were performed using GraphPad Prism 7, version 7.04 (GraphPad Software, Inc.). Works perfectly with little optimization. The TaqMan Mouse Immune Array from Applied Biosystems is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Lyophilized Standard. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Detects 144 Mouse Cytokines. VeriPlex Mouse Cytokine 9-Plex ELISA is a multiplex kit that has been developed to simultaneously quantitate Type I and II IFNs (IFN-α, IFN-β, IFN-γ), and other key cytokines released upstream and downstream of interferon signaling, including IL-1α, IL- 1β, IL-6, IL-10, RANTES and TNF-α. Q-Plex plates are built by adsorbing 4 distinct capture antibodies in a defined array to the bottom of each well. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet. It comes as 1 kit including 2 separate membranes labelled C3 and C4 (see below for images showing the array layout on each membrane). is a mediator and potential interventional target for NASH and subsequent liver cancer, Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output, Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine, Comparison of the immune response during acute and chronic Staphylococcus aureus infection, IL-23 secreted by myeloid cells drives castration-resistant prostate cancer, Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood, Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation, Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis, T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells, Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulatingadipose stromal interleukin 6 production in mice, Pharmacological HIF-inhibition attenuates postoperative adhesion formation, Depletion of microglia exacerbates postischemic inflammation and brain injury, Metabolic origins of spatial organization in the tumor microenvironment, Effective combinatorial immunotherapy for castration-resistant prostate cancer, Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection, A Jak1/2 inhibitor, baricitinib, inhibits osteoclastogenesis by suppressing RANKL expression in osteoblasts in vitro, Map3k8 controls granulocyte colony-stimulating factor production and neutrophil precursor proliferation in lipopolysaccharide-induced emergency granulopoiesis, A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer, Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity, Evaluation of PLGA containing anti-CTLA4 inhibited endometriosis progression by regulating CD4+CD25+Treg cells in peritoneal fluid of mouse endometriosis model, Novel Resolvin D2 Receptor Axis in Infectious Inflammation.